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. 2021 Apr 13;9:665089. doi: 10.3389/fcell.2021.665089

FIGURE 6.

FIGURE 6

Inhibition of the p38 MAPK pathway rescued disrupted steroidogenesis. (A) Western blot analysis of phospho-p38 (p-p38) and BMI1 in MLTC-1 cells treated with PTC-209 (5 μM), SB203580 (10 μM), or siRNA targeting Map3k3 (50 nM) for 48 h. (B) Quantification of (A). (C) Quantification of (A). (D) Immunofluorescence staining for p-p38 in MLTC-1 cells treated as indicated for 48 h. PTC-209, SB203580, and Map3k3 siRNA were used at the concentrations of 5 μM, 10 μM, and 50 nM, respectively. (E) The assessment of testosterone levels in MLTC-1 cells treated as indicated for 48 h. PTC-209, SB203580, and Map3k3 siRNA were used at the concentrations of 5 μM, 10 μM, and 50 nM, respectively. Testosterone levels were determined by ELISA after treatment with 1 IU/mL human chorionic gonadotropin (hCG) for 6 h. Scale bar, 20 μm. *p < 0.05.