TABLE 3.
Numbers (expressed as the mean and standard error of the mean) of oral pathogens [×105 colony-forming units (CFU)/mL] detected by absolute quantification PCR throughout the whole culturing duration on different samples, using specific primers and probes aimed to the 16S rRNA gene.
| Bacterial species | Incubation time (hours) | Blank | Cp-Ti | Ti-0.125G |
| Mean (SEM) (× 105 CFU/mL) | ||||
| S. mutans | 12 h | 53.6 (12.2) | 29.3 (11.1) | 15.1 (3.7) |
| 24 h | 29.1 (3.2) | 34.5 (2.8) | 23.2 (7.3) | |
| 48 h | 35.4 (6.9) | 15.4 (9.7) | 8.0 (0.4) | |
| 72 h | 19.6 (6.9) | 9.5 (1.2) | 6.7 (1.6) | |
| 96 h | 14.7 (4.5) | 11.3 (3.4) | 4.7 (1.4) | |
| F. nucleatum | 12 h | 120.9 (67.3) | 138.7 (32.5) | 53.4 (33.8) |
| 24 h | 279.7 (101.9) | 319.8 (80.0) | 138.9 (71.3) | |
| 48 h | 304.5 (176) | 102.3 (46.0) | 4.7 (1.0) | |
| 72 h | 147.7 (33.3) | 21.3 (9.0) | 2.6 (1.0) | |
| 96 h | 177.7 (161.5) | 8.2 (2.8) | 1.6 (0.4) | |
| P. gingivalis | 12 h | 157.4 (127.3) | 121.3 (52.8) | 77.13 (32.47) |
| 24 h | 324.3 (246.0) | 373.2 (210.4) | 46.76 (17.99) | |
| 48 h | 336.2 (262.5) | 193.1 (91.2) | 4.2 (3.2) | |
| 72 h | 315.6 (244.3) | 27.1 (16.9) | 4.2 (2.9) | |
| 96 h | 294.0 (206.0) | 10.6 (5.0) | 0.4 (0.2) | |