Figure 1.

Generation of sympathoadrenal-specific tyrosine hydroxylase (Th) KO mouse.A, schematic representation of the generation sympathoadrenal-specific Th KO mice. Th^fl/fl mice, in which exons 6 to 9 of the Th gene, are flanked with loxP sites (open arrowhead), were crossed with dopamine β-hydroxylase (DBH) Cre-ERT2 mice, which express the Cre-ERT2 recombinase under the 5.8-kb promoter region of human DBH gene, to generate mice Th^fl/fl and DBH Cre-ERT2 mice harboring loxP-flanked Th alleles. After administration of tamoxifen (TAM), the Th alleles are deleted in Cre-ERT2–expressing cells of DBH Cre-ERT2/Th^fl/fl mice (conditional KO [cKO]) but not of Th^fl/fl mice (Ctrl). B, schematic diagram of the experimental schedule. Eight- to 12-week-old male mice were intraperitoneally injected with 1 mg of TAM for 5 consecutive days. The mice were analyzed at 2 or 4 weeks after the last TAM injection.