Figure 2.

Immunohistochemical analysis of the adrenal gland and heart after administration of tamoxifen (TAM).A, tissue sections of the adrenal gland of Th^fl/fl (Ctrl) and dopamine β-hydroxylase Cre-ERT2/Th^fl/fl (conditional KO [cKO]) mice were prepared after 2 or 4 weeks after TAM injection (2 and 4 weeks, respectively). Tyrosine hydroxylase (TH) and aromatic amino acid decarboxylase (AADC) were visualized by immunofluorescent staining. Merged images were pseudocolored with TH (green) and AADC (magenta). Immunoreactivity of TH was decreased in slices of the adrenal gland from cKO mice compared with those of Ctrl mice. The scale bar represents 100 μm. B, tissue sections of the heart from Ctrl and cKO mice after 2 or 4 weeks after TAM injection. TH-positive nerve bundles (indicated by an arrow) in the surface of the heart from cKO mice were thinner than those of Ctrl mice. The scale bar represents 50 μm.