Figure 5.

Alterations in the tyrosine hydroxylase (TH) protein contents and the levels of noradrenaline in the tissues. Tissue homogenates were subjected to Western blot analysis for quantitation of the TH protein and β-actin in the Th^fl/fl (Ctrl) and dopamine β-hydroxylase Cre-ERT2/Th^fl/fl (conditional KO [cKO]) at 2 or 4 weeks after tamoxifen injection (2 and 4 weeks, respectively). GAPDH was used as a loading control. A–F, quantitation of the TH protein in the heart (A), pancreas (B), stomach (C), lung (D), spleen (E), and kidney (F). The blue circles indicate Ctrl mouse, orange circles indicate cKO mouse, and the bars indicate the means. The band intensities of TH were represented as the ratio of Ctrl mice in 2 and 4 weeks, separately. n = 5 in each group, except for Ctrl and cKO at 4 weeks of heart, n = 4. ∗∗p < 0.01; ∗∗∗p < 0.05, Welch's two-tailed t test corrected by Holm's method. G–L, the contents of noradrenaline (NA) in the heart (G), pancreas (H), stomach (I), lung (J), spleen (K), and kidney (L). n = 5 in each group, except for Ctrl and cKO at 4 weeks of heart, n = 4. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001, Tukey–Kramer test. M–R, the correlations between the NA contents and the TH protein amounts in the heart (M), pancreas (N), stomach (O), lung (P), spleen (Q), and kidney (R). The y-axis represents the NA contents, and the x-axis does the TH contents. Lines indicate the linear fitting, blue triangles indicate Ctrl mouse, and orange triangles indicate cKO mouse. Pearson's correlation coefficients were shown in the plots.