Figure 11.

Sequential magnification depicts the punctate distribution of excitatory synapses on the dendrites of hippocampal pyramidal neurons

(A–C) Indicate pyramidal neurons counter-labeled for post synaptic proteins, namely, PSD95 and Shank2 marked by Alexa 488 and Alexa 555, respectively. (C) Pseudocolor overlay of PSD95 (green) and Shank2 (red). The colocalized regions are marked in yellow.

(D–F) The inset presented in (C) is represented at higher magnification with PSD95 in (D) and Shank2 in (E) with the corresponding pseudocolor overlay in (F).

(G–I) Magnified images of the pseudocolor overlay marked by white regions in (F). Scale bar indicates 80 μm at (C), 15 μm at (F) and 3.5 μm at (I). Images were obtained using confocal microscopy.