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. 2021 Apr 14;2(2):100470. doi: 10.1016/j.xpro.2021.100470
PCR cycling conditions
Steps Temperature Time Cycles
Initial Denaturation 95°C 3 min 1
Denaturation 95°C 30 sec 30–35 cycles
Annealing 55°C 15 sec
Extension 72°C 30 sec
Final Extension 72°C 10 min 1
Hold 4°C forever