Fig. 2.

In vitro Schwann cells adhesion and migration on ECHs. (A) Live/dead assay of the SCs cultured on the hydrogel for one day. The green and red staining represent the live and dead cells, respectively. (B) The CCK-8 assay indicated that the cell viability exceeded 80% for the hydrogels after seeding (n = 5). (C) Cytoskeleton images demonstrated the adhesion of SCs cultured on each sample. (D) Wound-healing migration assay of the SCs on the hydrogels and control slide. (E) Quantitative analysis of the gap area at the denuded site (n = 3). (*p < 0.05, **p < 0.01, and ***p < 0.001). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)