Fig. 3.

Axonal outgrowth on the ECHs. (A) Immunocytochemistry of the PC-12 cells for the NF (green) and cell nuclei (blue) to analyze the axonal extension. (B) WB analysis to detect the NF and GAP43 expression values in PC-12 cells cultured in hydrogels and control conditions for 3 days. (C) Quantification of the integrated density for the protein bands (n = 3). (D–E) qRT-PCR data on the axonal gene expressions of the PC-12 cells. (F) Stained DRG for NF (green) and nuclei with DAPI (blue). (G–H) Analysis of the axonal lengths and distribution of the DRG for each group (n = 3). (*p < 0.05, **p < 0.01, and ***p < 0.001). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)