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Attenuation of gastrocnemius muscle atrophy after ECH treatment. (A) Gross images of the isolated gastrocnemius muscles in each group. (B) The cross-sectional view of the ipsilateral muscles highlighted by Masson's trichrome stain. (C–E) Statistical analysis of the (C) wet weight ratio of the gastrocnemius muscle, (D) percentage of muscle fiber, and (E) percentage of collagen deposits area (n = 3) (*p < 0.05, **p < 0.01, and ***p < 0.001).
