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. 2021 Apr 13;12:639104. doi: 10.3389/fpls.2021.639104

FIGURE 6.

FIGURE 6

Silencing of GhERF12 in cotton increased sensitivity to salinity. (A) Relative abundance of GhERF12 transcripts in GhERF12-silenced (TRV:ERF12) and control (TRV:00) plants were analyzed with qRT-PCR after 10 days inoculation. GhActin9 was used as an internal control. Data represent means ± SD (n = 6) from three independent replicates. (B) Length of primary roots in GhERF12-silenced and control plants treated with 100 mM NaCl were measured at the indicated time. (C) DAB and Trypan blue stain analysis were used to quantify ROS accumulation and cell death after salinity stress. (D) Survival rate of roots from GhERF12-silenced and control plants. (E) GhERF12-silenced and control adult plants (30-day old) treated with 250 mM NaCl for 7 days. (F) Root length and (G) number of lateral roots in GhERF12-silenced and control adult plants. (H) Activity of SOD and (I) POD in GhERF12-silenced and control plants after 250 mM NaCl treatment for 8 h. The data are means ± SD (n = 10). The asterisk (∗) indicate significant differences at P < 0.05 (t-test). All experiments were performed in triplicate.