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. 2021 Apr 22;15:11779322211011140. doi: 10.1177/11779322211011140

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© The Author(s) 2021

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 3. — A phylogenetic tree showing evolutionary relationship of the target protein (yellow marked) with other Tae4 proteins. The tree was generated using neighbor joining method based on BLOSUM62 scoring matrix by Jalview software. The values indicate percentage mismatches between 2 nodes (branch length). The target protein along with 3 other Shigella Tae4 amidases (shaded) seems to share the most recent common ancestor with E. coli amidases (blue color) rather than Salmonella enterica amidases (black color).