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. 2021 Apr 13;12:674415. doi: 10.3389/fmicb.2021.674415

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Copyright © 2021 Zhang, Li, Wang, Li, Ma, Chen, Li, Yang, Wang and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) showing the expression and the purification of the viral proteins using N-terminal His-tag. (A) Cef, Pin, MRH, Y00H, Y00G, and DexA proteins were collected in the soluble fraction, evident by the presence of bands of correct molecular weight in the elusion fraction. (B) MotB, RpbA, SegF, Y01A, ComCα, and Valyl proteins were expressed into the inclusion body, evident by the absence of bands of correct molecular weight in the elusion fraction and the presence of the bands in the insoluble fraction. M, Marker; FT, Flow-through fraction; W, Wash fraction; E, elusion fraction; Ins, Resolublized cell debris containing insoluble protein.