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. 2021 Apr 13;11:610885. doi: 10.3389/fonc.2021.610885

Figure 1.

Figure 1

Growth of OC cells is dependent on FASN. (A) Down-regulation of FASN protein expression by transfection of FASN siRNAs (30nM) was proven by Western blotting (left, one of three independent experiments) and was found to correlate with reduction of cell growth in A2780 cells (right). Results were obtained after 72 hours of incubation and are expressed in % of Control. Means ± SD, n = 3. One-tailed Student’s t-test at p < 0.01 (**) between cells transfected with non-targeting control siRNA (Control) or with FASN-targeting siRNA (FASN). (B) The FASN inhibitors G28UCM and Fasnall dose-dependently inhibit in vitro growth of A2780, OVCAR3 and SKOV3 OC cell lines after 72 hours of drug exposure. Note that concentrations of G28UCM are on a logarithmic, while Fasnall concentrations are on a linear scale. Formazan dye assay, means ± SD, n = 3. (C) Western blot analysis of dose- and time-dependent induction of apoptosis by G28UCM in A2780 OC cells as demonstrated by expression of PARP1 cleavage products (◄). Actin was used as loading control. The band intensities were determined using ImageJ software and the ratios between FASN (both bands in the left panel in (A) marked with]) or PARP1 cleavage products (◄ in C) and actin were given under the FASN (A) or PARP1 (C) autoradiographs. One of two independent determinations is shown.