Figure 2.

Expression of lipid metabolic proteins in OC cell lines as determined by Western blotting. (A) Baseline levels of the lipogenic enzymes ATP-citrate lyase (ACLY), acetyl-CoA carboxylase 1 (ACC1), ACC2 and FASN, the lipid transport proteins FABP1 – FABP9, and the lipid receptor proteins CD36 and LDLR in A2780, OVCAR3 and SKOV3 OC cell lines. A2780 cells were found to express the widest range of these lipid metabolic proteins. (B) Inhibition of FA synthesis by the FASN inhibitors G28UCM or Fasnall does not affect the expression of the lipid metabolic proteins in A2780 OC cells. Blots were (co-)probed for several proteins of interest and re-probed for actin as loading control. Previous antibodies were stripped from the membranes before detection of ACLY, ACC1 and ACC2. The band intensities were determined using ImageJ software, and in A the ratios between the proteins of interest and the corresponding actin are given under each autoradiograph, whereas in B these ratios were normalized to the untreated control samples (100%) and are expressed in percent of control. Experiments were repeated at least once.