Figure 4.

A 48-hours knock-down of FASN by specific siRNAs inhibits the uptake of exogenously supplied FA. Comparison of the data in the presence of 5%, 1% or 0% serum reveals that reduction of concurrent lipid contained in the serum can support the uptake of the fluorescent dodecanoic FA analog BODIPY 500/512 C1, C12 in the untreated control cells, but cannot reverse drug-mediated reduction of FA uptake. Fluorescence signals were normalized to cell numbers that have been determined in parallel by formazan dye cell proliferation assays and were expressed in % of vehicle(0.1% DMSO)-treated control cells grown in the presence of 5% serum. Means ± SD, n = 3. One-tailed Student’s t-test at p < 0.05 (*) and p < 0.01 (**) between cells transfected with FASN-targeting siRNA (FASN) or with non-targeting control siRNA (Control) grown in the presence of the corresponding concentration (5%, 1% or 0%) of serum.