Figure 1.

Chlamydia muridarum infection down-regulates miR-135a. C57BL/6 mice (n = 3) were intravaginally challenged with Cm (5 × 10⁴ IFUs in SPG buffer) or SPG buffer alone (mock). Genital tract tissue was collected at day 6 post-challenge and miR-135a expression was measured. Cell suspensions of naïve mouse genital tract and fibroblast cell line (McCoy) cells were exposed to Cm (2 x 10⁵ IFUs, MOI = 1) for 24 hours in a 24-well microplate. Relative expression of miR135a in Cm infected tissue/cells compared to the respective uninfected tissue/cells was determined by qRT-PCR and calculated using the 2−ΔΔCT method. Data shown are representative of 3 experiments. *p < 0.05 Student t test.