Figure 2.

miR-135a affects dendritic cell mediated Cm-primed CD4⁺ T cell proliferation and CCR5 gene expression. Bone marrow derived dendritic cells (BMDC) were stimulated with media alone, or media containing miR-135a mimic for 24 hours followed by exposure to Cm (MOI = 1). Cm primed BMDCs were co-cultured with splenic CD4⁺ T cells from day 6-post intravaginally challenged mice (5 x 10⁴ IFU) or mock infected. (A) Effect of miR-135a mimic on CD4⁺ T cell proliferation. (B) Effect of miR-135a mimic on CCR5 gene expression. Data shown are representative of 3 experiments. *p < 0.05 Student t test.