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. 2021 Apr 13;12:657548. doi: 10.3389/fpls.2021.657548

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Copyright © 2021 Takahashi, Betsuyaku, Okuzumi, Kiyosue and Hirakawa.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MpCIK weakly associates with MpCLV1 and MpTDR in N. benthamiana. (A) Schematic illustration of expressed receptors. (B) Co-immunoprecipitation experiment using anti-HA affinity matrix. The indicated combinations of MpCIK-3HS, MpCLV1-3FLAG and MpTDR-3FLAG constructs, together with p19 silencing suppressor, were transiently expressed in N. benthamiana. Total proteins were extracted and immunoprecipitated with anti-HA affinity matrix. Immunoblot analyses were performed using anti-HA or anti-FLAG antibody. In the presence of MpCIK-3HS, MpCLV1-3FLAG and MpTDR-3FLAG were co-precipitated with anti-HA affinity matrix. This experiment was repeated twice with similar results.