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Western blot of healthy control serum and early stage HGSOC serum diluted at 1:300 with 7 antigens in Validation I study. A. Secondary antibody anti-HIS tag IgG loading control. B. Secondary antibody anti-human IgG. Scans quantified on Odyssey software; background-corrected integrated intensity of anti-human IgG antibody (IRDye800) normalized as ratio to anti-His tag antibody (IRDye700) per antigen.
