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. 2021 Apr 26;6(1):e000711. doi: 10.1136/tsaco-2021-000711

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© Author(s) (or their employer(s)) 2021. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/.

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(A) Glycocalyx shedding in human umbilical vein endothelial cell (HUVEC) monolayers. ELISA kits were used to quantitate syndecan-1 and hyaluronic acid (HLA) levels present in the perfusate collected from HUVEC exposed to hypoxia (Hyp) (1% O₂) and epinephrine (epi) perfusion for 90 min. (B) HUVEC glycocalyx thickness measurement. Three-dimensional XYZ image stacks were acquired and processed and analyzed using Volocity cellular imaging and analysis software to assess glycocalyx thickness.