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. 2021 Apr 13;12:655551. doi: 10.3389/fphar.2021.655551

FIGURE 2.

FIGURE 2

2-APB or Si-TRPM7 reduces SNP-induced chondrocyte apoptosis (A) Hoechst 33,258 stain of 2-APB on SNP-induced chondrocyte chromatin of the nucleus (n = 3) (B) Rh123 satin of 2-APB on SNP-induced mitochondrial membrane potential (n = 3) (C) Annexin V-FITC and PI satin of 2-APB on SNP-induced chondrocyte apoptosis (n = 3) (D) Quantitative analysis of chondrocyte apoptosis rate (n = 3) (E) Western blot analysis of 2-APB on the Bcl-2/Bax ratio (n = 3) (F) Western blot analysis of 2-APB on cleaved PARP protein expression (n = 3) (G) qRT-PCR analysis of TRPM7 mRNA expression in chondrocytes treated with Si-TRPM7 (n = 3) (H) Western blot analysis of TRPM7 protein expression in chondrocytes treated with Si-TRPM7 (n = 3) (I) MTT analysis of Si-TRPM7 on cell viability of SNP-treated chondrocytes (n = 6) (J) Morphology of Si-TRPM7 on SNP-induced chondrocytes (n = 3) (K) Hoechst 33,258 stain of Si-TRPM7 on SNP-induced chondrocyte chromatin of the nucleus (n = 3) (L) Rh123 satin of Si-TRPM7 on SNP-induced mitochondrial membrane potential (n = 3) (M) Annexin V-FITC and PI satin of Si-TRPM7 on SNP-induced chondrocyte apoptosis (n = 3) (N) Quantitative analysis of chondrocyte apoptosis rate (n = 3) (O) Western blot analysis of Si-TRPM7 on Bcl-2/Bax ratio (n = 3) (P) Western blot analysis of Si-TRPM7 on cleaved PARP protein expression (n = 3). Chondrocytes were seeded in 6-well plates cultured for 24 h, then treated with 2-APB (100 μM) and SNP (0.5 mM) for 12 h, or chondrocytes were seeded in 6-well plates, cultured for 12 h, after transfected with Si-TRPM7 for 4 h and cultured for 24 h, then SNP (0.5 mM) was added and incubated for 12 h. Values are presented as mean ± SEM. * P < 0.05, ** p < 0.01, compared with control group; # p < 0.05, ## p < 0.01, compared with SNP group.