Table II.

Details of the primers used in this study.

Gene	Accession number	Forward primer	Reverse primer	Efficiency, %
GAPDH	NM_001289726.1	5’-AGGTCGGTGTGAACGGATTTG-3’	5’-TGTAGACCATGTAGTTGAGGTCA-3’	102
β-actin	NM_007393.5	5’-CCTCTATGCCAACACAGTGC-3’	5’-CCTGCTTGCTGATCCACATC-3’	110
ACP5	NM_001102405.1	5’-CAAAGAGATCGCCAGAACCG-3’	5’-CCTGCTTGCTGATCCACATC-3’	92
CALCR	NM_001042725.1	5’-ACTCCAGTTCTTCAGGCTCC-3’	5’-AGTCAGGGCAGTGCTGATAG-3’	109
CTSK	NM_007802.4	5’-ACTCCAGTCAAGAACCAGGG-3’	5’-TCAGAGTCAATGCCTCCGTT-3’	97
NFATc1	NM_016791.4	5’-CAACGCCCTGACCACCGATAG-3’	5’-GGCTGCCTTCCGTCTCATAGT-3’	104
ALPL	NM_001287172.1	5’-AGAAGTTCGCTATCTGCCTTGCCT-3’	5’-TGGCCAAAGGGCAATAACTAGGGA-3’	108
RUNX2	NM_001146038.2	5’-GACTGTGGTTACCGTCATGGC-3’	5’-ACTTGGTTTTTCATAACAGCGGA-3’	96
Col1a1	NM_007742.4	5’-GCTCCTCTTAGGGGCCACT-3’	5’-CCACGTCTCACCATTGGGG-3’	105
BGLAP	NM_007541.3	5’-TAGCAGACACCATGAGGACCATCT-3’	5’-CCTGCTTGGACATGAAGGCTTTGT-3’	96
SPP1	NM_001204201.1	5’-AGCAAGAAACTCTTCCAAGCAA-3’	5’-GTGAGATTCGTCAGATTCATCCG-3’	102
Akt1	NM_009652.3	5’-CTGCCCTTCTACAACCAGGA-3’	5’-CATACACATCCTGCCACACG-3’	93
Akt2	NM_001110208.2	5’-CTTCGGCAAGGTCATTCTGG-3’	5’-TTGAGGGCTGTAAGGAAGGG-3’	101
Akt3	NM_011785.4	5’-AGCCCAACCTCACAGATTGA-3’	5’-GTGCCACTTCATCCTTTGCA-3’	98

ACP5, acid phosphatase 5, tartrate resistant; Akt, AKT serine/threonine kinase; ALPL, alkaline phosphatase; BGLAP, bone gamma-carboxyglutamate protein; CALCR, calcitonin receptor; Col1a1, collagen type I alpha 1 chain; CTSK, cathepsin K; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; NFATC1, nuclear factor of activated T cells 1; RUNX2, RUNX family transcription factor 2; SPP1, secreted phosphoprotein 1.