Table 2. Effect of MAVA on the mechanical properties of myofibrils from rabbit fast skeletal in the 0–1 mM [Pi] range.
| Rabbit fast skeletal | PMAVA/P0 | kTR MAVA/kTR | kMAVA−/kTR |
|---|---|---|---|
| 5 µM MAVA | |||
| 0 Pi | 0.48 ± 0.05 (4) | 0.35 ± 0.03 (4) | 0.48 ± 0.07 (4) |
| Nominal Pi | 0.62 ± 0.02 (3) | 0.24 ± 0.03 (3) | 0.40 ± 0.06 (3) |
| 1 mM Pi | 0.52 ± 0.02 (5) | 0.36 ± 0.03 (5) | 0.63 ± 0.07 (5) |
| 10 µM MAVA | |||
| 0 Pi | 0.49 ± 0.05 (4) | 0.39 ± 0.06 (4) | 0.52 ± 0.05 (4) |
| Nominal Pi | 0.44 ± 0.06 (9) | 0.30 ± 0.02 (5) | 0.45 ± 0.04 (9) |
| 1 mM Pi | 0.30 ± 0.03 (4) | 0.56 ± 0.11 (4) | 0.51 ± 0.05 (4) |
Each group of data was collected from 5 and 10 µM MAVA-jump experiments in different rabbit fast skeletal myofibril batches in Ctrl conditions (Nominal Pi ∼170 μM) or the presence of increased (1 mM Pi) or enzymatically reduced (0 Pi) Pi concentration. All values are given as mean ± SEM; values in parentheses are the myofibril numbers. MAVA index refers to parameters measured in 5 or 10 µM MAVA, as indicated. The one way-ANOVA analysis revealed no systematic differences between each data set.