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. 2021 Apr 23;153(7):e202012811. doi: 10.1085/jgp.202012811

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© 2021 Rasicci et al.

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Figure 5. — ADP-release from actomyosin. Representative fluorescence transients of ADP release experiments using mantADP or pyrene actin. (A) We monitored mantADP dissociation by mixing acto–M2β-S1 complexed with mantADP with saturating ATP (1 mM), and the fluorescence transients were fit to a single exponential function (WT, k_obs = 228.3 ± 3.8 s^–1, and K104E, k_obs = 297.5 s^–1). (B) We examined ADP dissociation by mixing pyrene acto–M2β-S1.ADP with saturating ATP (2 mM), and the fluorescence transients were fit to a single exponential function (WT, k_obs = 215.8 ± 8.9 s^–1, and K104E, k_obs = 145.0 ± 2.5 s^–1).