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. 2021 Apr 23;153(7):e202012811. doi: 10.1085/jgp.202012811

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© 2021 Rasicci et al.

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Figure S1. — SDS-PAGE of RLC exchange. Representative SDS-PAGE gel of protein preparation run in precast NuPage Novex 4–12% Bis-Tris, 1.0 mm, 10- or 12-well gels, demonstrating quality of M2β-S1 purification and RLC exchange. Lanes were loaded with the following samples: (1) mol wt marker (BioRad Precision Plus Protein Standard: 250, 150, 100, 75, 50, 37, 25, 20, 15, and 10 kD, respectively), (2) stripped endogenous mRLC, (3) M2β-S1 WT human RLC (hRLC), (4) M2β-S1 K104E hRLC, (5) His-tagged hRLC control, and (6) M2β-S1 nonexchanged control. See labels for S1, endogenous murine RLC (mRLC) and ELC (mELC), as well as the exchanged hRLC. Note differential migration between His-tagged hRLC (19,589 D) and mRLC (18,864 D).