Table 1. Summary of steady-state ATPase results for human M2β-S1 exchanged with K104E versus WT RLC.
| Steady-state ATPase values (± SD), n = 3 | WT | K104E | WT Non-ex |
|---|---|---|---|
| v0 (s–1) | 0.02 ± 0.02 | 0.04 ± 0.04 | 0.02 ± 0.01 |
| kcat (s–1) | 4.5 ± 0.6 | 5.4 ± 0.8 | 5.4 ± 1.3 |
| KATPase (µM) | 49 ± 11 | 57 ± 14 | 53 ± 22 |
KATPase, the actin concentration at which the ATPase rate is one half maximal actin-activated ATPase activity; kcat, maximal actin-activated ATPase activity; v0, absence of actin; Non-ex, non-exchanged myosin with endogenous RLC.