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. 2021 Apr 26;218(6):e20201329. doi: 10.1084/jem.20201329

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© 2021 Amgen Inc

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Figure 7. — Gene expression and STARTRC analysis of CD4⁺ effector clusters. (A) Heatmaps of all CD4⁺ clusters C14-C20. (B) Violin plots of key marker genes. (C) Clonal expansion, cross-tissue migration, and phenotypic transition scores for CD4⁺ clusters quantified by STARTRAC for each animal (n = 4). Significance calculated by ANOVA. (D) Cross-cluster phenotypic transition scores between CD4⁺ clusters for all animals combined. P values calculated by permutation of clusters to determine significance of pairwise cluster sharing. ***, P < 0.001; **, P < 0.01; *, P < 0.05.