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. 2021 Apr 30;220(6):e202103105. doi: 10.1083/jcb.202103105

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© 2021 Li et al.

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Figure S1. — VMP1 deficiency does not impact early LD formation. (A) Confocal images of mCherry-tagged endogenous PLIN3 and BODIPY-positive LD puncta in HeLa WT or SKO cells treated with control or VMP1 siRNAs for 24 h followed by starvation in 1% lipoprotein-deficient serum for 16 h. Images were taken at a 2-min interval for up to 18 min after oleate addition (400 µM). (B) Quantification of the numbers of PLIN3- and BODIPY-positive LD puncta shown in A at indicated time points. The value of pairs indicates overlapped PLIN3 and BODIPY puncta. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 (two-way ANOVA, mean ± SD, n = 15–20 cells). Scale bars = 10 µm. Ctrl, control.