Skip to main content
. 2021 Apr 23;220(5):e202001188. doi: 10.1083/jcb.202001188

Table 1. Primers used for cloning in this study.

Plasmid cloning Primer Sequence
GFP-Ub-VPS13D EGFP-Ub forward 5′-TCT​AGA​TAA​CTG​ATC​ATA​ATC​AGC​CAT​AC-3′
GFP-Ub-VPS13D EGFPC1 forward 5′-GAT​CAC​TCT​CGG​CAT​GGA​C-3′
GFP-Ub-VPS13D EGFPC1 reverse 5′-CAT​TTT​ATG​TTT​CAG​GTT​CAG​GG-3′
GFP-Ub-VPS13D Ub-Vps13D forward 5′-TAC​TCC​GTC​TCA​GAG​GTG​GGA​TGT​TGG​AAG​GCC​TTG​TAG​C-3′
GFP-Ub-VPS13D Ub-Vps13D reverse 5′-ATT​ATG​ATC​AGT​TAT​CTA​GAT​CAG​GAG​TCC​AGC​TCC​AGC​TG-3′
GFP-Ub-VPS13DΔC Ub-Vps13D-dC reverse 5′-ATT​ATG​ATC​AGT​TAT​CTA​GAT​CAA​ATT​GGT​GTT​CCA​CCT​TG-3′
GFP-Ub-VPS13DΔN Ub-Vps13D-dN forward 5′-TAC​TCC​GTC​TCA​GAG​GTG​GGA​TGG​AGG​AGA​AAT​GGA​AGA​ATG​ACC-3′
GFP-Ub-VPS13DΔN Ub-Vps13D-dN reverse 5′-ATT​ATG​ATC​AGT​TAT​CTA​GAT​CAG​GAG​TCC​AGC​TCC​AGC​TG-3′
GFP-Ub-VPS13DmutG1200D Vps13D-G1200D forward 5′-AGT​ATA​GGT​GAC​ACC​AAA​GTT​AAT​GTC​TCA​ATG-3′
GFP-Ub-VPS13DmutG1200D Vps13D-G1200D reverse 5′-ACT​TTG​GTG​TCA​CCT​ATA​CTT​GCA​GTT​G-3′
GFP-Ub-VPS13DmutG4149S Vps13D-G4149S forward 5′-CCT​TGT​AGC​CAG​CAT​CCA​TGG​CCT​GGC​TC-3′
GFP-Ub-VPS13DmutG4149S Vps13D-G4149S reverse 5′-CAT​GGA​TGC​TGG​CTA​CAA​GGT​GTT​CAC​C-3′
GFP-Ub-VPS13DmutL2900S Vps13D-L2900S forward 5′-GGG​TGC​ACT​TCG​TGG​TTT​GCC​ACC​CTG​AC-3′
GFP-Ub-VPS13DmutL2900S Vps13D-L2900S reverse 5′-GCA​AAC​CAC​GAA​GTG​CAC​CCC​GTG​TGG​T-3′
GFP-Ub-VPS13DmutN34107I Vps13D-N4107I forward 5′-GGA​GTA​TCA​ATC​TCT​GCT​GCC​AAG​TTT​GC-3′
GFP-Ub-VPS13DmutN34107I Vps13D-N4107I reverse 5′-TTG​GCA​GCA​GAG​ATT​GAT​ACT​CCG​TGT​GTA​ACA​TTT​C-3′
GFP-Ub-VPS13DmutN3521S Vps13D-N3521S forward 5′-CGA​ATT​GAC​AGC​TTT​TCT​AAG​GTC​CCG​GTT​G-3′
GFP-Ub-VPS13DmutN3521S Vps13D-N3521S reverse 5′-TTA​GAA​AAG​CTG​TCA​ATT​CGG​AAA​GGA​G-3′
GFP-Ub-VPS13DmutR4228Q Vps13D reverse4228Q forward 5′-CAG​AGG​GTT​CAG​AAA​CCG​CGT​TGC​TGC​AC-3′
GFP-Ub-VPS13DmutR4228Q Vps13D reverse4228Q reverse 5′-CGC​GGT​TTC​TGA​ACC​CTC​TGT​GCT​TGA​G-3′
GFP-Ub-VPS13DmutS405R Vps13D_S405R forward 5′-TTA​GTG​AAC​CGT​CAG​ATC​CGC​TAG​CGC​TAC​CGG​TCG​CCA​CCA​T-3′
GFP-Ub-VPS13DmutS405R Vps13D_S405R reverse 5′-AGA​CAT​GAT​CTC​GCT​TTG​TAT​ACG​TGT​TCA​TAC​ACG​AGG​CAT​C-3′
GFP-Ub-VPS13DmutT1118M Vps13D-T1118M forward 5′-AAT​CCA​GAG​ATG​ATT​GTG​GAG​CTA​ATT​GG-3′
GFP-Ub-VPS13DmutT1118M Vps13D-T1118M reverse 5′-TCC​ACA​ATC​ATC​TCT​GGA​TTG​AGG​ATA​ATA​TC-3′
GFP-Ub-VPS13DmutT865A Vps13D-T865A forward 5′-GAT​TGA​TTT​ATG​CTT​CAG​ATC​CCA​AAT​ATC​CAG-3′
GFP-Ub-VPS13DmutT865A Vps13D-T865A reverse 5′-GAT​CTG​AAG​CAT​AAA​TCA​ATC​GAC​GCT​C-3′
pBMNz-GFP-CAT pBMN-CAT_reverse 5′-CGC​GGT​ACC​GTC​GAC​TGC​AGA​ATT​CTC​ACA​GAT​TTG​CCT​TCT​CCC​TTG​C-3′
pBMNz-GFP-CAT pBMN-GFP reverse 5′-ATA​AAA​TCT​TTT​ATT​TTA​TCG​TCG​ACT​TAC​TTG​TAC​AGC​TCG​TC-3′
pBMNz-GFP-CAT pBMNeGFP-CAT_forward 5′-TAC​AAG​TCC​GGA​CTC​AGA​TCT​CGA​GGG​ATG​GCT​GAC​AGC​CGG​GAT​C-3′
pCDNA-VPS13A/C-mycHis pcDNA-3xHA-C forward 5′-ATC​CAG​CAC​AGT​GGC​GGC​CGC​TAC​CCA​TAC​GAT​GTT​CCT​GAC​TAT​GCG​GGC​TAT​CCC​TAT​GAC​GTC​CCG​GAC​TAT​GCA​GG-3′
pCDNA-VPS13A/C-mycHis pcDNA-3xHA-C reverse 5′-GGT​TTA​AAC​GGG​CCC​TCT​AGA​CTA​AGC​GTA​ATC​TGG​AAC​GTC​ATA​TGG​ATA​GGA​GCC​TGC​ATA​GTC​CGG​GAC​GTC​ATA​GG-3′
pCDNA-VPS13C-mycHis Vps13A forward 5′-TAC​TCC​GTC​TCA​GAG​GTG​GGA​TGG​TTT​TCG​AGT​CGG​TGG​T-3′
pCDNA-VPS13C-mycHis Vps13A reverse 5′-ATT​ATG​ATC​AGT​TAT​CTA​GAT​CAG​AGG​CTC​GGA​GAA​GGT​TCT​C-3′
pCDNA-VPS13C-mycHis Vps13C forward 5′-TAC​TCC​GTC​TCA​GAG​GTG​GGA​TGG​TGC​TGG​AGT​CGG​T-3′
pCDNA-VPS13C-mycHis Vps13C reverse 5′-ATT​ATG​ATC​AGT​TAT​CTA​GAT​TTA​AGA​TGG​CAA​TTG​GGG​TCT​GA-3′
pHAGE-IRES-GFP-SKL IRES-GFP forward 5′-TGA​AAA​ACA​CGA​TGA​TAA​TAT​GGC​CAC​AAC​CAT​GGT​GAG​CAA​GGG​CGA​G-3′
pHAGE-IRES-GFP-SKL pHAGE-GFP-SKL reverse 5′-GTA​ATC​CAG​AGG​TTG​ATT​AGG​ATC​CCT​ACA​GCT​TGG​AAC​CGG​ACT​TGT​ACA​GCT​CGT​CCA​TGC-3′
pHAGE-IRES-GFP-SKL pHAGE-linker forward 5′-CCT​CCA​TAG​AAG​ACA​CCG​GCG​GCC​GCG​CTA​GCC​TCG​AGT​CTA​GAG​TCG​ACA​CGC​GTG​AAT​TCC​GCC​CCC​CCC​CCC​TAA​CGT-3′
pHAGE-IRES-GFP-SKL pHAGE-linker reverse 5′-ACG​TTA​GGG​GGG​GGG​GGC​GGA​ATT​CAC​GCG​TGT​CGA​CTC​TAG​ACT​CGA​GGC​TAG​CGC​GGC​CGC​CGG​TGT​CTT​CTA​TGG​AGG-3′
pHAGE-PEX14-IRES-GFPSKL pHAGE-PEX14 forward 5′-CAT​AGA​AGA​CAC​CGG​CGG​CCG​CGC​TAG​CCA​CCA​TGG​CGT​CCT​CGG​AGC​AG-3′
pHAGE-PEX14-IRES-GFPSKL pHAGE-PEX14 reverse 5′-TTA​GGG​GGG​GGG​GGC​GGA​ATT​CAC​GCG​TGT​CGA​CCT​AGT​CCC​GCT​CAC​TCT​CG-3′
pHAGE-PEX16-IRES-GFPSKL pHAGE-PEX16 forward 5′-CAT​AGA​AGA​CAC​CGG​CGG​CCG​CGC​TAG​CCA​CCA​TGG​AGA​AGC​TGC​GGC​TC-3′
pHAGE-PEX16-IRES-GFPSKL pHAGE-PEX16 reverse 5′-TTA​GGG​GGG​GGG​GGC​GGA​ATT​CAC​GCG​TGT​CGA​CTC​AGC​CCC​AAC​TGT​AGA​AG-3′
pHAGE-PEX19-IRES-GFPSKL pHAGE-PEX19 forward 5′-CAT​AGA​AGA​CAC​CGG​CGG​CCG​CGC​TAG​CCA​CCA​TGG​CCG​CCG​CTG​AGG​AA-3′
pHAGE-PEX19-IRES-GFPSKL pHAGE-PEX19 reverse 5′-TTA​GGG​GGG​GGG​GGC​GGA​ATT​CAC​GCG​TGT​CGA​CTC​ACA​TGA​TCA​GAC​ACT​GTT​CAC​CAC
pHAGE-PEX26-IRES-GFPSKL pHAGE-PEX26 forward CAT​AGA​AGA​CAC​CGG​CGG​CCG​CGC​TAG​CCA​CCA​TGA​AGA​GCG​ATT​CTT​CGA​CC-3′
pHAGE-PEX26-IRES-GFPSKL pHAGE-PEX26 reverse 5′-TTA​GGG​GGG​GGG​GGC​GGA​ATT​CAC​GCG​TGT​CGA​CTC​AGT​CAC​GGA​TGC​GGA​G-3′
pHAGE-PEX3-IRES-GFPSKL PEX3-IRES reverse 5′-TTA​GGG​GGG​GGG​GGC​GGA​ATT​CAC​GCG​TGT​CGA​CTC​ATT​TCT​CCA​GTT​GCT​G-3′
pHAGE-PEX3-IRES-GFPSKL pHAGE-PEX3 forward 5′-CAT​AGA​AGA​CAC​CGG​CGG​CCG​CGC​TAG​CCA​CCA​TGC​TGA​GGT​CTG​TAT​GG-3′