Figure 4.

Time-dependent changes in the transcriptional regulatory network in chelidonine-treated pancreatic cancer cells. After chelidonine treatment, (A) BxPC-3 cells and (B) MIA PaCa-2 cells were seeded in a black 96-well plate and the expressions of several transcription factors were investigated by measuring the fluorescence intensity every 12 hours for 2 days in cells transduced with lentiviral vector containing transcription factor binding sites. The transcriptional regulatory network was inferred using BTNET. The color of each circle in A indicates the change in expression level of each transcription factor (red for up-regulation and green for down-regulation) and the arrow between circles indicates edge information (dotted line for inference and solid line for prior knowledge).