Table 1.

TRAbs levels assessed by two different techniques

Controlled sample	TRAbs level (IU/L)
	automated immunoassay (E411, Roche Diagnostics, Mannheim, Germany) reference range <1.75 IU/L	second-generation immunoassay (Thermo Fisher Brahms TRAb human RIA) reference range <1.5 IU/L
Mother	3.2	3.86
Neonate (10 days)	1.7	*
Neonate (5 weeks)	0.4	<1
Neonate (7 weeks)	<0.3	<1
Neonate (18 weeks)	<0.8**	<1

^*Second-generation immunoassay result could not be obtained for this sample due to an insufficient serum volume.

^**New reagent formulation (modification of the limit of quantification).