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. 2021 Apr 15;148(7):dev197319. doi: 10.1242/dev.197319

Fig. 2.

Fig. 2.

YY1 promotes Notch1 expression in thymocytes. (A-E) All Yy1f/f Mx1-cre and Mx1-Cre mice were treated with four doses of pI-pC and evaluated 3-7 days after the last injection. (A) Flow cytometry gating strategy and quantification of Notch1+ cells in DN1 (Lin−CD44+CD25−), DN2 (Lin−CD44+CD25+), DN3 (Lin−CD44−CD25+) and DN4 (Lin−CD44−CD25−) T cells. Samples without Notch1 antibody staining were used as the negative control. (B) Evaluation of Notch1 MFI in DN T cells. Representative flow plot (left) and quantification of Notch 1 MFI (right). (C) Western blot and quantification to detect cleaved Notch1 expression in total thymocytes. Note that the control β-actin blot is reproduced from Fig. 1B as YY1 was detected on the same membrane. (D) RT-qPCR to detect Notch1 and Hes1 transcript levels. (E) YY1 occupancy at Notch1 locus based on ChIP-seq. n represents the number of mice. Data are presented as mean±s.e.m. **P<0.01, ***P<0.001, ****P<0.0001 (two-tailed unpaired Student's t-test).