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. 2021 Apr 15;148(7):dev197319. doi: 10.1242/dev.197319

Fig. 3.

Fig. 3.

YY1 is required for DN1 transition and early T cell survival ex vivo. (A-E) Yy1f/f and Yy1f/f Vav-Cre fetuses were harvested on E14.5. Lin− fetal liver cells were isolated and were co-cultured with OP9-DL1 feeder cells. Cells were harvested for flow analysis on day 10. (A) Representative flow gating strategy for DN1 (Lin−CD44+CD25−), DN2 (Lin−CD44+CD25+), DN3 (Lin−CD44−CD25+) and DN4 (Lin−CD44−CD25−). (B) Quantification of percentage of each DN population. Two-tailed unpaired Student's t-test. (C) Quantification of absolute cell number of each DN population. Two-tailed unpaired Student's t-test. (D) Representative flow gating strategy for early apoptotic (annexin V+DAPI−), late apoptotic (annexin V+DAPI+) and non-apoptotic (annexin V−DAPI−) cells. (E) Quantification of non-apoptotic, early apoptotic and late apoptotic cells in DN1 population. Two-way ANOVA. n represents the number of mice; Data are presented as mean±s.e.m. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001.