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. 2021 Apr 15;148(7):dev197319. doi: 10.1242/dev.197319

Fig. 4.

Fig. 4.

A lineage-specific requirement for YY1 PcG function/REPO domain in adult hematopoiesis. (A) Experimental strategy: Yy1f/f Mx1-Cre bone marrow cells transduced with MigR1, MigR1-FlagYY1 or MigR1-FlagYY1ΔREPO, or Mx1-Cre bone marrow cells transduced with MigR1, were injected into lethally irradiated CD45.1+ congenic mice. At 4 weeks after BMT, recipient mice were treated with pI-pC injections and endogenous YY1 was deleted. Peripheral blood chimerism of BMT recipient mice was assessed 4 and 20 weeks after BMT. At 20 weeks post-BMT, bone marrow cells and thymocytes from reconstituted mice were harvested for flow analysis. (B) Before BMT, flow analysis of percentage of GFP+ cells and GFP MFI of transduced bone marrow cells at 24 h post-viral infection. One-way ANOVA. (C) Western blot to detect exogenous Flag-YY1, Flag-YY1ΔREPO and endogenous YY1 expression in GFP+ peripheral lymphocytes after pI-pC injections. Quantification of exogenous Flag-YY1, Flag- YY1ΔREPO and endogenous YY1 protein expressions. (D) Lineage evaluation of peripheral blood T cells (Thy1.2+CD19−), B cells (Thy1.2−CD19+), monocytes (Mac1+Gr1−) and neutrophils (Mac1+Gr1+) at week 4 and week 20 post-BMT. Two-way ANOVA. (E) Lineage evaluation of bone marrow T cells (Thy1.2+CD19−), B cells (Thy1.2−CD19+), monocytes (Mac1+Gr1−) and neutrophils (Mac1+Gr1+) at week 20 post-BMT. One-way ANOVA. n represents the number of mice. Data are presented as mean±s.e.m. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001.