Fig. 5.

YY1 REPO domain/PcG function is required for DN1 transition and Notch1 cell surface expression. (A) Quantification of percentage and absolute number of CD45.2⁺GFP⁺ thymocytes. (B) Representative gating strategy in retroviral BMT mice. Thymocytes were gated for CD45.2⁺GFP⁺Lin⁻, and then further gated for DN1 (CD45.2⁺GFP⁺Lin⁻CD44⁺CD25⁻), DN2 (CD45.2⁺GFP⁺Lin⁻CD44⁺CD25⁺), DN3 (CD45.2⁺GFP⁺Lin⁻CD44⁻CD25⁺), DN4 (CD45.2⁺GFP⁺Lin⁻CD44⁻CD25⁻) and c-Kit⁺ DN1 (CD45.2⁺GFP⁺Lin⁻c-Kit⁺CD44⁺CD25⁻). Lineage markers include CD4, CD8, B220, Ter 119, Gr1, Mac1 and NK1.1. Lineage gating strategy was included in Fig. S6. (C) Quantification of percentage and absolute number of DN1, DN2, DN3, DN4 and c-Kit⁺ DN1 cells. (D) Representative flow gating and quantification of Notch1 MFI in DN1, DN2 and DN3 populations. n represents the number of mice. Data are presented as mean±s.e.m. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001 (one-way ANOVA).