Figure 2: SARSNotch can detect Spike protein with high sensitivity when surface expressed in a model of infected cells.

A) Schematic of experimental setup. K562 sender cells expressing the Spike ectodomain on their surface, modeling infected cells that express Spike on the cell surface, were co-cultured with sentinel cells expressing SARSNotch and BFP downstream of SARSNotch activation. B) Density estimates for Jurkat sentinel cell activation after co-culture with no, untransformed, or Spike-expressing K562 cells at equal density for 72 hours. C) Percent of activated Jurkat sentinel cells as a function of the ratio of K562 cells per sentinel cell after 72 hours of co-culture. D) Percent of activated sentinel T-cells as a function of the ratio of K562 cells per sentinel after 72 hours of co-culture. Data points and error bars represent mean and standard deviation of 3 biological replicates.