Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Primers for RT-qPCR.
| Gene name | Product size (bp) | TM (°C) | Amplification efficiency | Primer sequences (5′ to 3′) |
|---|---|---|---|---|
| GAPDH | 141 | 60 | 97% | F: GGAGCTGCCCAGAACATTATC |
| R: GCAGGTCAGGTCCACGACA | ||||
| ANPEP | 79 | 60 | 96% | F: CAGCACAGCCTGCTCCTA |
| R: GTCGGTGTTCTGCCAGTGT | ||||
| ENPEP | 78 | 60 | 96% | F: GGGACAGGTGCTATGGAGAA |
| R: GGCAGACTCGTTGGGATCAT | ||||
| UPP1 | 96 | 60 | 98% | F: GGAGCCAAGATGGTGACTATC |
| R: CCATACCGTGACTGACTGACAA | ||||
| SLC6A19 | 99 | 60 | 97% | F: CCAAGATGCCTGTCTCACCTT R: CAAGCACACCTTCCATGTTTCC |
