Fig. 5.

Effect of aloe-emodin on the viability of colon cancer cells. a Cell viability was determined by MTT assay after treated with aloe-emodin on NCM460 for 24 h and HCT116 for 24 h, 48 h and 72 h. *P < 0.05 versus Control. b Colony formation experiments of HCT116 colon cancer cells treated with aloe-emodin for 24 h, 48 h. **P < 0.01 versus Control. c Fluorescence microscope to observe the cell morphology of HCT116 cells treated with aloe-emodin for 48 h (× 20 magnification). All the experimental data were collected from at least three independent experiments. Two-tailed Student’s t-test (a), one-way ANOVA (b)