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. 2021 Apr 27;21:238. doi: 10.1186/s12935-021-01942-8

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Effect of aloe-emodin on apoptosis of HCT116 colon cancer cells. a DAPI staining (× 20 magnification), 40 μM 80 μM for 24 h (× 40 magnification) and the white arrow points to the saient features of apoptotic cells. b The cell cycle is assessed by flow cytometry. ***P < 0.001 versus Control. ^###P < 0.001 versus 20 μM. c Capase-3 activity assay. Data are expressed as mean ± SD. * P < 0.05, ***P < 0.001 versus Control. ^#P < 0.05 versus 20 μM. All the experimental data were collected from at least three independent experiments. Multiple comparisons using LSD method (b), Tamhane' T2 and one-way ANOVA (c)