Fig. 7.

Treatment with aloe-emodin for 24 h and western blotting analysis the protein expression levels. Aloe-emodin activated cleaved-caspase 3, cleaved-caspase 9, and significantly decreased Bcl-2 expression, ultimately causing the release of cytochrome c from the mitochondria into the cytoplasm. All the experimental data were collected from at least three independent experiments. Data are expressed as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 versus Control. ^#P < 0.05, versus 20 μM (Multiple comparisons using LSD method)