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. 2021 Apr 26;21:235. doi: 10.1186/s12935-021-01934-8

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Knockdown of PSMC2 inhibited cell growth in vitro. PSMC2 knockdown cell models were constructed through the transfection of shCtrl or shPSMC2. a The knockdown efficiencies of PSMC2 in PC-3 and DU 145 cells were detected by qPCR. b The successful knockdown of PSMC2 in PC-3 and DU 145 cells were confirmed by western blotting. c The effects of PSMC2 knockdown on cell proliferation of PC-3 and DU 145 cells were evaluated by Celigo cell counting assay. d Colony formation assay was performed to detect the effects of PSMC2 on colony formation ability of PC-3 and DU 145 cells. Results were presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001