Fig. 3.

SIRT-1 regulates HepG2 intracellular lipid and ROS crosstalk with OA. The cells were pretreated with T6 (2 μM) or CAY (20 μM) 2 h, followed incubated with OA (1.5 mM) for 48 h. a The cells were stained oil red O. b Immunofluorescence detected intracellular ROS. Scale bars are 50 μm and 10 μm respectively