Table 3.
The primers used for PCR for resistance genes in S. aureus isolates
| Primer name | Sequence (5’-3’) |
Annealing temperature(℃) | Size (bp) |
References |
|---|---|---|---|---|
| blaZ -F | AACACCTGCTGCTTTCGGTA | 55.5 | 314 | This study |
| blaZ- R | CACTCTTGGCGGTTTCACTT | |||
| mecA-F | CTTTGCTAGAGTAGCACTCG | 55.5 | 533 | Herold B.C., et al. |
| mecA-R | GCTAGCCATTCCTTTATCTTG | |||
| ermA-F | CTACACTTGGCTTAGGATGA | 56.5 | 311 | This study |
| ermA-R | AGTGACTAAAGAAGCGGTAA | |||
| ermB-F | TAACGACGAAACTGGCTAA | 56.0 | 414 | This study |
| ermB-R | CTGTGGTATGGCGGGTAA | |||
| ermC-F | GAGGCTCATAGACGAAGAAA | 54.5 | 375 | This study |
| ermC-R | AAGTTCCCAAATTCGAGTAA | |||
| aacA-aphD-F | ATTGAAGATTTGCCAGAACA | 56.5 | 178 | This study |
| aacA-aphD-R | CACTATCATAACCACTACCG |
F forward primer, R reverse primer