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NKX6-1 mediates the activation of stemness, SHH, and Notch signaling pathways. qRT-PCR analysis of Notch, WNT and SHH signaling. a qRT-PCR for NOTCH1, NOTCH2, WNT1, FZD1, SFRP5, SHH, IHH, and DHH in NKX6-1-overexpressing MES-SA cells. b qRT-PCR for NOTCH1, NOTCH2, and SHH in NKX6-1 knockdown MES-SA/DX5 cells. c Expression of SHH c and NOTCH d downstream genes was determined by qRT-PCR. *p < 0.05
