Skip to main content
. Author manuscript; available in PMC: 2021 Sep 23.
Published in final edited form as: Neuron. 2020 Jul 15;107(6):1124–1140.e11. doi: 10.1016/j.neuron.2020.06.027

Figure 6: Loss of C9ORF72 protein or DPRs do not alter the nuclear expression of POM121.

Figure 6:

(A) Maximum intensity projections from SIM imaging of Nups in nuclei isolated from control and C9orf72−/− iPSNs. Genotype as indicated on left, antibodies as indicated on top. (B) Quantification of Nup spots and volume. N = 1 control and 1 C9orf72 null line, differentiations conducted in triplicate, 50 NeuN+ nuclei per line/differentiation. Two-way ANOVA with Tukey’s multiple comparison test was used to calculate statistical significance. (C) SIM imaging of POM121 in nuclei isolated from wildtype iPSNs overexpressing GFP tagged Poly(GA), Poly(GR), and Poly(PR) DPRs. Overexpression as indicated on left, antibodies and time points as indicated on top. (D-E) Quantification of percent total nuclear volume occupied by POM121 2 days (D) and 7 days (E) after overexpression of DPRs. N = 3 wildtype iPSC lines, 50 GFP+ nuclei per line/overexpression. One-way ANOVA with Tukey’s multiple comparison test was used to calculate statistical significance. Scale bar = 5 μm.