IHC	MSP	PSQ	qMSP	Bead array	MS‐MLPA	PCR‐HRM	PCR‐mRNA	Other
IHC	None	Felsberg 2009 (1 vs 1) Hsu 2015 (1 vs 2) Karayan‐Tapon 2010 (1 vs 1) Lalezari 2013 (1 vs 1) Lechapt‐Zalcman 2012 (1 vs 1) Melguizo 2012 (1 vs 1) Quillien 2014 (test) (1 vs 1) Yang 2012 (1 vs 1)	Hsu 2017 (1 vs 1) Karayan‐Tapon 2010 (1 vs 6) Kristensen 2016 (1 vs 1) Lalezari 2013 (1 vs 1) Quillien 2014 (test) (1 vs 32)	Hsu 2017 (1 vs 2) Karayan‐Tapon 2010 (1 vs 1) Quillien 2014 (test) (1 vs 1)	None	None	Quillien 2014 (test) (1 vs 1)	Felsberg 2009 (1 vs 1) Karayan‐Tapon 2010 (1 vs 1)	Kristensen 2016 (1 vs 3)
MSP	—	Barbagallo 2014 (2) Hsu 2017 (2) Lattanzio 2015 (2)	Barbagallo 2014 (2 vs 2) Havik 2012 (1 vs 9) Hsu 2017 (2 vs 1) Karayan‐Tapon 2010 (1 vs 6) Kim 2016 (1 vs 1) Lalezari 2013 (1 vs 1) Lattanzio 2015 (1 vs 2) McDonald 2013 (1 vs 1); Quillien 2014 (test) (1 vs 32)	Havik 2012 (1 vs 2) Hsu 2017 (2 vs 2) Karayan‐Tapon 2010 (1 vs 1) Quillien 2014 (test) (1 vs 1)	Bady 2012 (M‐GBM) (1 vs 1)	Park 2011 (1 vs 2)	Havik 2012 (1 vs 1) Quillien 2014 (test) (1 vs 1) Yamashita 2018 (1 vs 5)	Felsberg 2009 (1 vs 1) Karayan‐Tapon 2010 (1 vs 1)	Thon 2017 (1 vs 1)
PSQ	—	—	Barbagallo 2014 (2) Brigliadori 2016 (2) Chai 2018 (7‐site cohort) (3) Chai 2018 (8‐site cohort) (3) Dunn 2009 (6) Havik 2012 (9) Karayan‐Tapon 2010 (6) Lattanzio 2015 (2) Quillien 2014 (test) (32) Quillien 2014 (validation) (3) Quillien 2016 (12)	Havik 2012 (9 vs 2) Hsu 2017 (1 vs 2) Karayan‐Tapon 2010 (6 vs 1) Quillien 2014 (test) (32 vs 1) Quillien 2016 (12 vs 5)	Bady 2012 (E‐GBM) (1 vs 2)	None	Havik 2012 (9 vs 1) Quillien 2014 (test) (32 vs 1)	Karayan‐Tapon 2010 (6 vs 1)	Barault 2015 (1 vs 1) Dahlrot 2018 (NS Cohort) (1 vs 1) Dahlrot 2018 (RSD Cohort) (1 vs 1) Kristensen 2016 (1 vs 3)
qMSP	—	—	—	Havik 2012 (2) Hsu 2017 (2) Nguyen 2015 (2) Quillien 2016 (5) Yoshioka 2018 (5)	None	None	Havik 2012 (2 vs 1) Quillien 2014 (test) (1 vs 1)	Karayan‐Tapon 2010 (1 vs 1)	Bell 2017 (1 vs 1)
Bead array	—	—	—	—	Bady 2012 (E‐GBM) (1)	None	None	None	None
MS‐MLPA	—	—	—	—	—	Park 2011 (2)	None	None	None
PCR‐HRM	—	—	—	—	—	—	Yamashita 2018 (5)	None	None
PCR‐mRNA	—	—	—	—	—	—	—	None	None
Other	—	—	—	—	—	—	—	—	Almuqate 2018 (2) Kristensen 2016 (3)
IHC: immunohistochemistry; MS‐MLPA: methylation‐specific multiplex ligation‐dependent probe amplification; MSP: methylation‐specific polymerase chain reaction; PCR‐HRM: polymerase chain reaction with high‐resolution melting; PCR‐mRNA: polymerase chain reaction‐messenger ribonucleic acid; PSQ: pyrosequencing; qMSP: quantitative methylation‐specific polymerase chain reaction.