Figure 1.

AtPiezo responds to mechanical stimulation. (a) Histochemical GUS staining is shown in four-day-old seedling of pAtPiezo::GUS lines. The plants that vertically grew on the 1% (w/v) agar medium are shown at left (Vertical). The plants that horizontally grew for 24 h followed by vertical growth for three days on the medium are shown at right (Horizontal). Scale bars = 2 mm. The corresponding root tips are shown in the bottom panels. Scale bars = 0.1 mm. (b) Graph showing the mean GUS intensity of different root tips. Measurements of GUS gray value within a 0.1 mm × 0.1 mm rectangular area in the root tips. Data are presented as mean ± SD (n = 15) and asterisks indicate a significant difference (Unpaired t-test, P value < .05). (c) Expression of AtPiezo in four-days-old WT roots. Roots were collected under different conditions and AtPiezo mRNA levels were measured by qRT-PCR. UBQ10 was used as a normalizer. Data are means ± SD, and asterisks indicate a significant difference (Unpaired t-test, P value < .05). (d) Histochemical GUS staining is shown in four-day-old seedlings of pAtPiezo::GUS lines. The plants that vertically grew in the 0.8% (w/v) agar medium with non-helical roots are shown at left (Non-Helical). The plants with helical roots are shown at right (Helical). Scale bars = 2 mm. The corresponding root tips are shown in the bottom panels, Scale bars = 0.1 mm. (e) Graph showing the mean GUS intensity of different root tips. Measurements of GUS gray value within a 0.1 mm × 0.1 mm rectangular area in the root tips. Data are presented as mean ± SD (n = 15) and asterisks indicate a significant difference (Unpaired t-test, P value < .05). (f) Expression of AtPiezo in four-day-old WT roots. Roots were collected under different conditions and AtPiezo mRNA levels were measured by qRT-PCR. UBQ10 was used as a normalizer. Data are means ± SD, and asterisks indicate a significant difference (Unpaired t-test, P value < .05)