Figure2.

Heterologously expressed AtPiezo in Hela cells

The pcDNA3.1–AtPiezo constructs were transfected into Hela cells with Exfect Transfection Reagent. Cells were grown for 24 hours before analysis. All images were obtained using an excitation wavelength of 488 nm and detection wavelength of 505–535 nm by a confocal laser scanning microscope (Leica TCS SP8). All experiments were repeated at least three times and similar results were obtained each time.