Table 8.
Top 30 hypermethylated CpG sites (lowest P < 2.42E–03) located within gene body regions in PNS cows compared with Control cows at 5 years of age
| BTAa | Mb | Differenceb | Gene |
|---|---|---|---|
| 25 | 20.2 | 11.58 | Transmembrane protein 180 (LOC101905757) |
| 1 | 2.9 | 11.32 | Hormonally up-regulated neu-associated kinase (HUNK) |
| 21 | 10.8 | 15.93 | Nuclear receptor subfamily 2 group F member 2 (NR2F2) |
| 11 | 83.7 | 14.94 | LRAT domain containing 1 (LRATD1) |
| 23 | 51.6 | 11.87 | Forkhead box F2 (FOXF2) |
| 15 | 34.3 | 15.72 | CXADR like membrane protein (CLMP) |
| 6 | 23.8 | 14.77 | Solute carrier family 39 member 8 (SLC39A8) |
| 5 | 26.2 | 21.41 | Homeobox C12 (HOXC12) |
| 7 | 48.1 | 21.11 | Paired like homeodomain 1 (PITX1) |
| 18 | 64.9 | 12.84 | Zinc finger protein 772 (ZNF772) |
| 4 | 101.8 | 15.80 | Pleiotrophin (PTN) |
| 21 | 3.0 | 23.12 | ATPase phospholipid transporting 10A (Putative) (ATP10A) |
| 7 | 48.1 | 16.37 | Paired like homeodomain 1 (PITX1) |
| 28 | 45.4 | 15.89 | C-X-C motif chemokine ligand 12 (CXCL12) |
| 25 | 27.8 | 16.24 | Solute carrier family 5 member 2 (SLC5A2) |
| 18 | 19.7 | 13.90 | Spalt like transcription factor 1 (SALL1) |
| 13 | 73.3 | 29.53 | Junctophilin 2 (JPH2) |
| 24 | 46.0 | 13.84 | Sialic acid binding Ig like lectin 15 (SIGLEC15) |
| 21 | 61.2 | 12.40 | Goosecoid homeobox (GSC) |
| 18 | 8.8 | 11.69 | M-phase phosphoprotein 6 (MPHOSPH6) |
| 10 | 32.9 | 13.70 | Meis homeobox 2 (MEIS2) |
| 3 | 3.6 | 15.36 | Retinoid X receptor gamma (RXRG) |
| 7 | 48.7 | 11.05 | C-X-C motif chemokine ligand 14 (CXCL14) |
| 7 | 4.2 | 14.99 | Ceramide synthase 1 (CERS1) |
| 3 | 16.8 | 18.36 | Natriuretic peptide receptor 1 (NPR1) |
| 4 | 117.3 | 22.39 | Dipeptidyl peptidase like 6 (DPP6) |
| 11 | 105.9 | 16.92 | Glutamate ionotropic receptor NMDA type subunit 1 (GRIN1) |
| 11 | 98.1 | 10.29 | GTPase activating Rap/RanGAP domain like 3 (GARNL3) |
| 10 | 103.3 | 21.83 | Tetratricopeptide repeat domain 7B (TTC7B) |
| 4 | 76.7 | 14.89 | Insulin-like growth factor binding protein 1 (IGFBP1) |
aBTA: Bos taurus autosome. DNA methylation in bolded chromosomes was located within exons, otherwise within introns.
bMethylation difference was calculated by subtracting the mean methylation ratio in Control at 5 years of age from the mean methylation ratio in PNS at 5 years of age.